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Manamohan, M.
- Cytochrome P450 Isoforms Transcriptional, Larval Growth and Development Responses to Host Allelochemicals in the Generalist Herbivore, Helicoverpa armigera (Hubner) (Lepidoptera:Noctuidae)
Abstract Views :221 |
PDF Views:82
Authors
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru 560 089, IN
2 Department of Biotechnology, St Martin’s Engineering College, Dulapally, Secunderabad 500 014, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru 560 089, IN
2 Department of Biotechnology, St Martin’s Engineering College, Dulapally, Secunderabad 500 014, IN
Source
Current Science, Vol 111, No 5 (2016), Pagination: 901-906Abstract
Helicoverpa armigera (Hubner) is a polyphagous pest causing severe yield loss in many important crops. Host plants produce allelochemicals to deter insect pests and in response, insects deploy cytochrome P450 monooxygenases (P450s) to detoxify allelochemicals. Understanding the response of P450s to allelochemical exposure is key to effective pest management. We studied the response of seven H. armigera P450 isoforms to different concentrations of three allelochemi-cals (gossypol, tomatine and xanthotoxin) and their effects on insect growth and survival. Allelochemicals strongly induced overexpression of some P450s. CYP6AE14 exhibited the highest overexpression in gossypol treatment. CYP6AE14 and CYP6B7 exhibited higher overexpression in xanthotoxin treatment and CYP6B7 showed the highest overexpression in tomatine treatment. Overall, CYP6AE14 and CYP6B7 were induced by all three allelochemicals. Higher (0.5 and 1.0 μg) concentrations of allelochemicals caused significant larval growth retardation. Interestingly, gossypol showed a hormetic effect, i.e. larval weight was approximately 10% higher at lower (0.025 μg) concentration. Highest larval mortality (53%) was observed in tomatine treatment. These findings would help in identifying suitable P450 isoforms in the management of H. armigera.Keywords
Allelochemicals, Cytochrome P450 Isoforms, Helicoverpa armigera, Pest Management, Real-Time PCR.- Application of Genome Editing In Entomology
Abstract Views :113 |
PDF Views:68
Authors
R. Asokan
1,
Anil Rai
2,
Sangeetha Dash
1,
M. Manamohan
1,
K. Ashok
1,
C. N. Bhargava
1,
Rohan Wishard
1,
Sanjay Kumar Pradhan
1,
M. S. Parvathy
1
Affiliations
1 ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru 560089, Karnataka, IN
2 ICAR-Indian Agricultural Statistics Research Institute, New Delhi 110012, IN
1 ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru 560089, Karnataka, IN
2 ICAR-Indian Agricultural Statistics Research Institute, New Delhi 110012, IN
Source
Indian Journal of Entomology, Vol 84, No S1 (2022), Pagination: 96-103Abstract
Genome editing mediated by programmable CRISPR/Cas system is a new, emerging technology that holds greater potential in developing genetic pest management strategies via precision guided sterile insect technique; genetic improvement of pollinators, natural enemies etc. The seemingly innocuous prokaryotic immunity has now been translated into a mammoth site specific editing technology for its use in medical, agricultural, diagnostic fields etc. Until recently most of the genome editing work was dependent on RNA directed site specific cleavage by Cas9 enzyme followed by error prone, non-homologous end joining (NHEJ) resulting in random mutations. Later development of improved editing systems like base editor and prime editor have enabled obtaining site-specific, pre determined mutations without a double stranded break. Similarly many engineered Cas9 variants and other Cas proteins belonging to different class and types such as Cas3, Cas12a, Cas13a have improved the existing editing tool box. Currently efforts are being made to design and implement suitable gene drive methods for various pests such as spotted wing Drosophila, Drosophila suzukii Matsumura, Diamondback moth, Plutella xylostella (Linnaeus) , many species mosquito, Med fly, Ceratitis capitata Wiedem etc..Keywords
CRISPR/Cas systems, genome editing, double strand break, guide RNA, gene knockout, insect pest management, PAM sequence, non-homologous pairingReferences
- Baena-lopez L A, Alexandre C, Mitchell A, Pasakarnis I, Vincent J P. 2013. Accelerated homologous recombination and subsequent genome modification. Drosophila Development 140: 4818-4825.
- Bassett A R, Tibbit C, Ponting C P, Liu J L. 2013. Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell Reports 4: 220-228.
- Cong I, Ran F A, Cox D, Lin S, et al. 2013, Multiplex genome engineering using CRISPR/Cas systems. Science 339(6121): 819-823.
- Dong S Z, Lin J Y, Held N L, Clem R J, et al. 2015. Heritable CRISPR/Cas9 mediated genome editing in the yellow fever mosquito, Aedes aegypti. PLoS One 10(3): e0122353. doi:10.1371/journal.pone.0122353
- Gaj T, Gersbach C A, Barbas C F. 2013. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in Biotechnology 31(7): 397-405.
- Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. Journal of Bacteriology 169 (12): 5429-5433.
- Ishino Y, Krupovic M, Forterre P. 2018. History of CRISPRCas from encounter with a mysterious repeated sequence
- to genome editing technology. Journal of Bacteriology . https://doi.org/10.1128/JB.00580-17
- Kistler K E, Vosshall L B, Matthews B J. 2015. Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti. Cell Reports 11(1): 51-60.
- Lander E S. 2016. The Heroes of CRISPR. Cell https://doi.org/10.1016/j.cell.2015.12.041
- Ledford H. 2015. Alternative CRISPR system could improve genome editing. Nature 526(7571): 17.
- Makarova K S, Wolf Y I, Iranzo J. 2020. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology 18: 67-83.
- Oye K A, Esvelt K, Appleton E, Catteruccia F, et al. 2014. Regulating gene drives Science 345 (6197): 626-628.